IDEAS home Printed from https://ideas.repec.org/a/caa/jnlvet/v66y2021i7id189-2020-vetmed.html
   My bibliography  Save this article

Optimisation of phagocytosis assay in rainbow trout (Oncorhynchus mykiss)

Author

Listed:
  • H Minarova

    (Department of Ecology & Diseases of Zoo Animals, Game, Fish and Bees, Faculty of Veterinary Hygiene and Ecology, University of Veterinary Sciences Brno, Brno, Czech Republic
    Department of Infectious Diseases and Preventive Medicine, Veterinary Research Institute, Brno, Czech Republic)

  • P Ondrackova

    (Department of Infectious Diseases and Preventive Medicine, Veterinary Research Institute, Brno, Czech Republic)

  • M Palikova

    (Department of Ecology & Diseases of Zoo Animals, Game, Fish and Bees, Faculty of Veterinary Hygiene and Ecology, University of Veterinary Sciences Brno, Brno, Czech Republic
    Department of Zoology, Fisheries, Hydrobiology and Apiculture, Faculty of AgriSciences, Mendel University in Brno, Brno, Czech Republic)

  • J Mares

    (Department of Zoology, Fisheries, Hydrobiology and Apiculture, Faculty of AgriSciences, Mendel University in Brno, Brno, Czech Republic)

  • J Blahova

    (Department of Animal Protection and Welfare & Veterinary Public Health, Faculty of Veterinary Hygiene and Ecology, University of Veterinary Sciences Brno, Brno, Czech Republic)

  • K Jarova

    (Department of Ecology & Diseases of Zoo Animals, Game, Fish and Bees, Faculty of Veterinary Hygiene and Ecology, University of Veterinary Sciences Brno, Brno, Czech Republic)

  • M Faldyna

    (Department of Infectious Diseases and Preventive Medicine, Veterinary Research Institute, Brno, Czech Republic)

Abstract

A phagocytosis assay is one of the most commonly used functional immunological methods. There are many possible ways of assessing leucocytes and their ability to ingest different particles. The aim of this study was to optimise the phagocytosis assay in rainbow trout (Oncorhynchus mykiss) using labelled zymosan particles (Alexa Fluor 488 and Texas Red conjugate). Whole blood was incubated with the particles under different conditions and leucocytes were subsequently isolated by haemolysis in a hypotonic environment. The effect of the different incubation time, temperature, blood volume and dilution on the phagocytic activity was evaluated by flow cytometry. Our experiments showed that the incubation for at least 2 h at 15 °C provided optimal results, while the blood volume and dilution had no significant effect. The optimised assay will be used for the examination of fish health and in further experimental studies.

Suggested Citation

  • H Minarova & P Ondrackova & M Palikova & J Mares & J Blahova & K Jarova & M Faldyna, 2021. "Optimisation of phagocytosis assay in rainbow trout (Oncorhynchus mykiss)," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 66(7), pages 298-304.
    • Handle: RePEc:caa:jnlvet:v:66:y:2021:i:7:id:189-2020-vetmed
    DOI: 10.17221/189/2020-VETMED
    as

    Download full text from publisher

    File URL: http://vetmed.agriculturejournals.cz/doi/10.17221/189/2020-VETMED.html
    Download Restriction: free of charge
    File URL: http://vetmed.agriculturejournals.cz/doi/10.17221/189/2020-VETMED.pdf
    Download Restriction: free of charge
    File URL: https://libkey.io/10.17221/189/2020-VETMED?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:caa:jnlvet:v:66:y:2021:i:7:id:189-2020-vetmed. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Ivo Andrle (email available below). General contact details of provider: https://www.cazv.cz/en/home/ .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.