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Effects of age and litter-of-origin on cryopreserved spermatozoa in Sumava rams

Author

Listed:
  • Anežka Málková

    (Department of Animal Science, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences, Prague, Prague - Suchdol, Czech Republic)

  • Martin Ptáček

    (Department of Animal Science, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences, Prague, Prague - Suchdol, Czech Republic)

  • Filipp Georgijevič Savvulidi

    (Department of Animal Science, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences, Prague, Prague - Suchdol, Czech Republic)

  • Szabolcs Tamás Nagy

    (Hungarian University of Agriculture and Life Sciences, Institute of Animal Sciences, Georgikon Campus, Keszthely, Hungary)

  • Luděk Stádník

    (Department of Animal Science, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences, Prague, Prague - Suchdol, Czech Republic)

Abstract

This study aimed to evaluate the influence of the internal factors of ram age and litter-of-origin on semen quality during the cryopreservation process in Sumava sheep rams. This breed is included in the protected genetic resources of the Czech Republic. The sires were systematically divided into four groups according to age (under 1.5 years, 1.5 to 2.5 years, 2.5 to 3.5 years, and over 4.5 years) and according to litter frequency (singletons vs twins). Semen was evaluated after equilibration, and after cryopreservation using iSperm® mCASA and flow cytometry. During cryopreservation, there was a significant decrease in total sperm motility by 53.5%, progressive motility by 38%, and cells with intact plasma membrane and acrosome by 47%. Frozen-thawed sperm kinematic parameters showed significant age-related variations, with rams aged 4.5 and older displaying notably higher total and progressive motility (16.2% and 6.24%, respectively). Rams born as twins exhibited 3.77% (P < 0.05) higher progressive motility and 5.5% (P < 0.05) higher total motility compared to those born as singles. The sperm of older rams (> 4.5 years) exhibited higher viability (10.1%) and lower damage to the plasma membrane after freeze-thawing (23.6%), (P < 0.05). Twins showed significantly higher sperm viability (4.98%, P < 0.05) than singletons. These rams produce a larger quantity of higher-quality insemination doses after cryopreservation. For Sumava rams, in particular, broadening the sire selection base helps to select suitable rams for breeding. As the sire ages, his genetic value within the production herd may decline with time. This contrasts with genetic resource protection, where the aim is to preserve and store as many high-quality semen samples as possible.

Suggested Citation

  • Anežka Málková & Martin Ptáček & Filipp Georgijevič Savvulidi & Szabolcs Tamás Nagy & Luděk Stádník, 2024. "Effects of age and litter-of-origin on cryopreserved spermatozoa in Sumava rams," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 69(4), pages 129-138.
    • Handle: RePEc:caa:jnlcjs:v:69:y:2024:i:4:id:32-2024-cjas
    DOI: 10.17221/32/2024-CJAS
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    References listed on IDEAS

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    1. Vojtěch Pešan & Zuzana Rečková & Martin Hošek & Radek Filipčík & Katarina Soušková & Tomáš Kopec & Martina Pešanová Tesařová, 2023. "Evaluation of crystallisation structures of cervical mucus in Zwartbles sheep with previous oestrus synchronisation," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 68(9), pages 383-390.
    2. Filipp Georgijevic Savvulidi & Martin Ptacek & Anezka Malkova & Jakub Beranek & Ludek Stadnik, 2021. "Optimizing the conventional method of sperm freezing in liquid nitrogen vapour for Wallachian sheep conservation program," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 66(2), pages 55-64.
    3. Chunyan Li & Qiuyue Liu & Xiangyu Wang & Wenping Hu & Deping Han & Joram Mwashigadi Mwacharo & Caihong Wei & Mingxing Chu & Ran Di, 2020. "Expression and localization of PIWI proteins in testis and ovary of domestic sheep," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 65(3), pages 86-96.
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