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Impact of selected antimitotic substances on doubled haploid and polyploid regeneration in microspore cultures of swede (Brassica napus ssp. napobrassica (L.) Hanelt)

Author

Listed:
  • Miroslav Klíma
  • Yamen H. Shmeit

    (Crop Research Institute, Prague-Ruzyně, Czech Republic
    Faculty of Tropical AgriSciences, Czech University of Life Sciences Prague, Prague, Czech Republic)

  • Pavel Kopecký

    (Centre of the Region Haná for Biotechnological and Agricultural Research, Crop Research Institute, Olomouc, Czech Republic)

  • Pavel Vítámvás

    (Crop Research Institute, Prague-Ruzyně, Czech Republic)

  • Klára Kosová

    (Crop Research Institute, Prague-Ruzyně, Czech Republic)

  • Ilja T. Prášil

    (Crop Research Institute, Prague-Ruzyně, Czech Republic)

  • Eloy Fernández-Cusimamani

    (Faculty of Tropical AgriSciences, Czech University of Life Sciences Prague, Prague, Czech Republic)

Abstract

Several antimitotic agents were tested in three embryogenic doubled haploid (DH) lines of swede (Brassica napus ssp. napobrassica). No effect on embryogenesis was observed at the given concentrations of colchicine (5 μmol/L or 50 mg/L) and treatment time of 24 hours. Flow cytometric analysis of microspore embryos revealed a significant increase in the percentage of DH compared to the control for all substances: ethalfluralin, 62.2%; trifluralin, 58.3%; colchicine, 56.1%; amiprophos-methyl, 54.4%; pronamide, 35.0%; control, 13.3%. The occurrence of pure tetraploids was low (0-5%), mixoploids - especially n+2n - were high in all treatments, including the control (29.4-71.1%). Their presence can be explained as a consequence of spontaneous diploidisation and/or endoreduplication during embryogenesis or embryo germination.

Suggested Citation

  • Miroslav Klíma & Yamen H. Shmeit & Pavel Kopecký & Pavel Vítámvás & Klára Kosová & Ilja T. Prášil & Eloy Fernández-Cusimamani, 2024. "Impact of selected antimitotic substances on doubled haploid and polyploid regeneration in microspore cultures of swede (Brassica napus ssp. napobrassica (L.) Hanelt)," Czech Journal of Genetics and Plant Breeding, Czech Academy of Agricultural Sciences, vol. 60(2), pages 79-85.
    • Handle: RePEc:caa:jnlcjg:v:60:y:2024:i:2:id:84-2023-cjgpb
    DOI: 10.17221/84/2023-CJGPB
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    References listed on IDEAS

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    1. Iva Smýkalová & Martina Větrovcová & Miroslav Klíma & Ivana Macháčková & Miroslav Griga, 2006. "Efficiency of Microspore Culture for Doubled Haploid Production in the Breeding Project "Czech Winter Rape"," Czech Journal of Genetics and Plant Breeding, Czech Academy of Agricultural Sciences, vol. 42(2), pages 58-71.
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    1. repec:caa:jnlcjg:v:preprint:id:84-2023-cjgpb is not listed on IDEAS
    2. Miroslav Klíma & Miroslava Vyvadilová & Vratislav Kučera, 2008. "Chromosome doubling effects of selected antimitotic agents in Brassica napus microspore culture," Czech Journal of Genetics and Plant Breeding, Czech Academy of Agricultural Sciences, vol. 44(1), pages 30-36.

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