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Construction of high-density genetic map and QTL mapping in Nicotiana tabacum backcrossing BC4F3 population using whole-genome sequencing

Author

Listed:
  • Zhijun Tong

    (Key Laboratory of Tobacco Biotechnological Breeding, National Tobacco Genetic Engineering Research Center, Yunnan Academy of Tobacco Agricultural Sciences, Kunming, Yunnan, P.R. China)

  • Sanjie Jiang

    (BGI Genomics, BGI-Shenzhen, Shenzhen, Guangdong, P.R. China)

  • Weiming He

    (BGI Genomics, BGI-Shenzhen, Shenzhen, Guangdong, P.R. China)

  • Xuejun Chen

    (Key Laboratory of Tobacco Biotechnological Breeding, National Tobacco Genetic Engineering Research Center, Yunnan Academy of Tobacco Agricultural Sciences, Kunming, Yunnan, P.R. China)

  • Lixin Yin

    (BGI Genomics, BGI-Shenzhen, Shenzhen, Guangdong, P.R. China)

  • Dunhuang Fang

    (Key Laboratory of Tobacco Biotechnological Breeding, National Tobacco Genetic Engineering Research Center, Yunnan Academy of Tobacco Agricultural Sciences, Kunming, Yunnan, P.R. China)

  • Yafei Hu

    (BGI Genomics, BGI-Shenzhen, Shenzhen, Guangdong, P.R. China)

  • Fangchan Jiao

    (Key Laboratory of Tobacco Biotechnological Breeding, National Tobacco Genetic Engineering Research Center, Yunnan Academy of Tobacco Agricultural Sciences, Kunming, Yunnan, P.R. China)

  • Chi Zhang

    (BGI Genomics, BGI-Shenzhen, Shenzhen, Guangdong, P.R. China)

  • Jianmin Zeng

    (Key Laboratory of Tobacco Biotechnological Breeding, National Tobacco Genetic Engineering Research Center, Yunnan Academy of Tobacco Agricultural Sciences, Kunming, Yunnan, P.R. China)

  • Xinfu Wu

    (Key Laboratory of Tobacco Biotechnological Breeding, National Tobacco Genetic Engineering Research Center, Yunnan Academy of Tobacco Agricultural Sciences, Kunming, Yunnan, P.R. China)

  • Shancen Zhao

    (BGI Institute of Applied Agriculture, BGI-Shenzhen, Shenzhen, Guangdong, P.R. China)

  • Jianbo Jian

    (BGI Genomics, BGI-Shenzhen, Shenzhen, Guangdong, P.R. China
    Department of Biotechnology and Biomedicine, Technical University of Denmark, Lyngby, Denmark)

  • Bingguang Xiao

    (Key Laboratory of Tobacco Biotechnological Breeding, National Tobacco Genetic Engineering Research Center, Yunnan Academy of Tobacco Agricultural Sciences, Kunming, Yunnan, P.R. China)

Abstract

Backcrossing is a powerful tool for plant breeding. The improved marker-assisted backcrossing intends to transfer targeted genes or quantitative trait loci (QTLs) of interest from a donor parent into a recurrent parent. In this study, a tobacco BC4F3 population was generated using Y3 and K326 as hybrid parents and YF1-1 as F1 parents. High-throughput sequencing data of 381 pedigree populations were used to construct high-density genetic maps containing 24 142 high-quality single nucleotide polymorphism (SNP) markers with an average genetic distance of 0.59 cM. A genome module analysis was then performed for all the offspring. A total of forty-three candidate QTLs for six agronomics traits were identified. This study provides original biomarkers for tobacco breeding and offers clues for prospective backcrossing applications in other plants.

Suggested Citation

  • Zhijun Tong & Sanjie Jiang & Weiming He & Xuejun Chen & Lixin Yin & Dunhuang Fang & Yafei Hu & Fangchan Jiao & Chi Zhang & Jianmin Zeng & Xinfu Wu & Shancen Zhao & Jianbo Jian & Bingguang Xiao, 2021. "Construction of high-density genetic map and QTL mapping in Nicotiana tabacum backcrossing BC4F3 population using whole-genome sequencing," Czech Journal of Genetics and Plant Breeding, Czech Academy of Agricultural Sciences, vol. 57(3), pages 102-112.
    • Handle: RePEc:caa:jnlcjg:v:57:y:2021:i:3:id:8-2021-cjgpb
    DOI: 10.17221/8/2021-CJGPB
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