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Study of informative DNA markers of the Rf1 gene in sunflower for breeding practice

Author

Listed:
  • Nikolay MARKIN

    (Southern Federal University, Rostov-on-Don, Russia)

  • Alexander USATOV

    (Southern Federal University, Rostov-on-Don, Russia)

  • Maksim MAKARENKO

    (Southern Federal University, Rostov-on-Don, Russia)

  • Kiril AZARIN

    (Southern Federal University, Rostov-on-Don, Russia)

  • Oleg GORBACHENKO

    (L.A. Zhdanov Don Experimental Station of the All-Russian Institute of Oil Plants, Oporny, Rostov Region, Russia)

  • Natalia KOLOKOLOVA

    (Southern Federal University, Rostov-on-Don, Russia)

  • Tatyana USATENKO

    (L.A. Zhdanov Don Experimental Station of the All-Russian Institute of Oil Plants, Oporny, Rostov Region, Russia)

  • Olga MARKINA

    (Southern Federal University, Rostov-on-Don, Russia)

  • Vera GAVRILOVA

    (N.I. Vavilov Institute of Plant Genetic Resources, St. Petersburg, Russia)

Abstract

The investigation of DNA markers associated with the pollen fertility restoration gene (Rf1) was conducted in Helianthus. Two sequence-characterized amplified region (SCAR) markers - HRG01 and HRG02 were informative for the identification of Rf1 gene in selections of sunflower plants. The codominant character of the HRG01 marker and HRG01 amplicon polymorphism has been determined. Five annual and twenty-six perennial species of sunflower were tested for the presence of Rf genotypes. HRG02 proved to be a more appropriate marker for Rf1 determination in perennial species, and HRG01 was more informative for annual species. We have also developed the multiplex RT-PCR test system, which allows simultaneously detecting the dominant allele of Rf1 and CMS-PET1 associated mitotype.

Suggested Citation

  • Nikolay MARKIN & Alexander USATOV & Maksim MAKARENKO & Kiril AZARIN & Oleg GORBACHENKO & Natalia KOLOKOLOVA & Tatyana USATENKO & Olga MARKINA & Vera GAVRILOVA, 2017. "Study of informative DNA markers of the Rf1 gene in sunflower for breeding practice," Czech Journal of Genetics and Plant Breeding, Czech Academy of Agricultural Sciences, vol. 53(2), pages 69-75.
    • Handle: RePEc:caa:jnlcjg:v:53:y:2017:i:2:id:108-2016-cjgpb
    DOI: 10.17221/108/2016-CJGPB
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