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Establishment of protocol to investigate the expression of Sucrose Phosphate Synthase 2 (SPS2) in Vietnamese golden melon (cucumis melo L.)

Author

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  • Nguyen Tran Dong Phuong

    (Ho Chi Minh City Open University, Ho Chi Minh City, Vietnam)

  • Tran Tan Dat

    (Ho Chi Minh City Open University, Ho Chi Minh City, Vietnam)

  • Thieu Hong Hue

    (Ho Chi Minh City Open University, Ho Chi Minh City, Vietnam)

  • Le Huyen Ai Thuy

    (Ho Chi Minh City Open University, Ho Chi Minh City, Vietnam)

  • Lao Duc Thuan

    (Ho Chi Minh City Open University, Ho Chi Minh City, Vietnam)

Abstract

The accumulation of soluble sugars, especially sucrose, in mature Vietnamese golden melon, has been reported to be the determining factor of the sweetness of fruits. The gene of SPS2 has been shown to be mainly involved in the metabolic signal pathway as those for the synthesis of sucrose. The purpose of the current study is to establish a procedure to investigate the expression of the gene of SPS2 in Cucumis melo L. The materials of melon’s exocarp and mesocarp tissues were applied to isolate the total of RNAs. The procedure of Real-time PCR within the primers of SPS-F and SPS-R was successfully established as seen through the results. As a result, the cycle threshold (Ct) of 27.19, and 28.12, respectively, were observed in the sample of exocarp and mesocarp tissues. For authenticity, the products were sequenced, as determined as the gene of SPS by BLAST (NCBI). In conclusion, the procedure of investigation of SPSs gene expression was successfully established in Cucumis melo L.

Suggested Citation

  • Nguyen Tran Dong Phuong & Tran Tan Dat & Thieu Hong Hue & Le Huyen Ai Thuy & Lao Duc Thuan, 2023. "Establishment of protocol to investigate the expression of Sucrose Phosphate Synthase 2 (SPS2) in Vietnamese golden melon (cucumis melo L.)," HO CHI MINH CITY OPEN UNIVERSITY JOURNAL OF SCIENCE - ENGINEERING AND TECHNOLOGY, HO CHI MINH CITY OPEN UNIVERSITY JOURNAL OF SCIENCE, HO CHI MINH CITY OPEN UNIVERSITY, vol. 13(2), pages 55-59.
    • Handle: RePEc:bjw:techen:v:13:y:2023:i:2:p:55-59
    DOI: 10.46223/HCMCOUJS.tech.en.13.2.2786.2023
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    Keywords

    cucumis; gene expression; melon; SPS2;
    All these keywords.

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