Milk Borne Pathogens: Isolation and Identification Health Risk to Population Especially Young Ones

Milk and milk made products because of their nutritive value are considered very important food for all consumers and especially for young ones. The consumers are at risk because of toxic chemicals and food borne pathogens in milk and infants, young children or the persons suffering with HIV/AIDS, cancer, diabetes, kidney disease, and transplant patients are at greater risk. In the present study for milk borne pathogens’ identification, DNA of bacterial isolates was amplified using universal set of forward primer pA 5’AGA GTT TGA TCC TGG CTC AG 3’ (828) and reverse primer pH 5’AAG GAG GTG ATC CAG CCG CA 3’( 1542 1522), corresponding to positions 828 and 15421522, respectively from Escherichia coli. 16s rRNA gene sequences were amplified and sequenced. Twelve microorganisms are identified as Salmonella enterica, Citrobacter koseri, Escherichia fergusonii, Klebsiella pneumonia, Serratia liquifaciens, Shigella flexneri, Kosakonia sacchari and Enterobacter species, Klebsiella vericola, Escherichia vulneris, and Escherichia coli strains on the basis of 16s rRNA sequences Submission to NCBI Gene Bank. Some of them have shown pathogenecity and resistance to some antibiotics, which should be properly identified further for risk analysis to consumers. We have also reported in our previous study the presence of carcinogens, phthalates and hormone disrupters in milk. Food adulterants, chemicals and pathogens presence are major causes for developing disorder in children which needs attention of scientific community.