IDEAS home Printed from
   My bibliography  Save this article

Gene Expression during the Cell Cycle: Obfuscation of Original Cell-Cycle Gene Expression Data by Normalization


  • Stephen Cooper

    (Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI 48109 5620, USA; 2The Center for Cell Cycle Analysis, 4430 Exeter Drive, M303, Longboat Key, FL 34228-2226)


Normalization of raw data on gene expression during the cell cycle obscures the original experimental data and makes it appear as if all genes have the same numerical level of cyclical expression. Results that would not support gene expression because of minimal variations thus appear, after normalization, to be stronger than they actually are. Consideration of the effect of normalization raises critical questions about many experiments on the cell cycle dependent variation of gene expression—that is, proposed cyclical changes in mRNA content—during the cell cycle.

Suggested Citation

  • Stephen Cooper, 2015. "Gene Expression during the Cell Cycle: Obfuscation of Original Cell-Cycle Gene Expression Data by Normalization," Journal of Cells, Conscientia Beam, vol. 1(1), pages 1-7.
  • Handle: RePEc:pkp:joucel:2015:p:1-7
    DOI: 10.18488/journal.97/2015.1.1/

    Download full text from publisher

    File URL:
    Download Restriction: no

    File URL:
    Download Restriction: no

    More about this item


    Access and download statistics


    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:pkp:joucel:2015:p:1-7. See general information about how to correct material in RePEc.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: (Editorial Office). General contact details of provider: .

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service hosted by the Research Division of the Federal Reserve Bank of St. Louis . RePEc uses bibliographic data supplied by the respective publishers.