Advanced Search
MyIDEAS: Login

Identification of Multiple Subtypes of Campylobacter jejuni in Chicken Meat and the Impact on Source Attribution

Contents:

Author Info

  • Megan L. Devane

    ()
    (Institute of Environmental Science and Research Limited, Christchurch Science Centre, P.O. Box 29-181, Christchurch 8041, New Zealand)

  • Brent J. Gilpin

    ()
    (Institute of Environmental Science and Research Limited, Christchurch Science Centre, P.O. Box 29-181, Christchurch 8041, New Zealand)

  • Beth Robson

    ()
    (Institute of Environmental Science and Research Limited, Christchurch Science Centre, P.O. Box 29-181, Christchurch 8041, New Zealand)

  • John D. Klena

    ()
    (University of Canterbury, Private Bag 4800, Christchurch 8041, New Zealand
    Current address: Centers for Disease Control and Prevention-Emerging Infectious Diseases, Beijing 100000, China)

  • Marion G. Savill

    (Institute of Environmental Science and Research Limited, Christchurch Science Centre, P.O. Box 29-181, Christchurch 8041, New Zealand
    Current address: Affordable Water Limited, 72 Argyle Street, Herne Bay, Auckland 1011, New Zealand)

  • John A. Hudson

    ()
    (Institute of Environmental Science and Research Limited, Christchurch Science Centre, P.O. Box 29-181, Christchurch 8041, New Zealand)

Registered author(s):

    Abstract

    Most source attribution studies for Campylobacter use subtyping data based on single isolates from foods and environmental sources in an attempt to draw epidemiological inferences. It has been suggested that subtyping only one Campylobacter isolate per chicken carcass incurs a risk of failing to recognise the presence of clinically relevant, but numerically infrequent, subtypes. To investigate this, between 21 and 25 Campylobacter jejuni isolates from each of ten retail chicken carcasses were subtyped by pulsed-field gel electrophoresis (PFGE) using the two restriction enzymes Sma I and Kpn I. Among the 227 isolates, thirteen subtypes were identified, the most frequently occurring subtype being isolated from three carcasses. Six carcasses carried a single subtype, three carcasses carried two subtypes each and one carcass carried three subtypes. Some subtypes carried by an individual carcass were shown to be potentially clonally related. Comparison of C. jejuni subtypes from chickens with isolate subtypes from human clinical cases ( n = 1248) revealed seven of the thirteen chicken subtypes were indistinguishable from human cases. None of the numerically minor chicken subtypes were identified in the human data. Therefore, typing only one Campylobacter isolate from individual chicken carcasses may be adequate to inform Campylobacter source attribution.

    Download Info

    If you experience problems downloading a file, check if you have the proper application to view it first. In case of further problems read the IDEAS help page. Note that these files are not on the IDEAS site. Please be patient as the files may be large.
    File URL: http://www.mdpi.com/2077-0472/3/3/579/pdf
    Download Restriction: no

    File URL: http://www.mdpi.com/2077-0472/3/3/579/
    Download Restriction: no

    Bibliographic Info

    Article provided by MDPI, Open Access Journal in its journal Agriculture.

    Volume (Year): 3 (2013)
    Issue (Month): 3 (September)
    Pages: 579-595

    as in new window
    Handle: RePEc:gam:jagris:v:3:y:2013:i:3:p:579-595:d:28898

    Contact details of provider:
    Web page: http://www.mdpi.com/

    Related research

    Keywords: multiple subtypes; chicken carcass; diversity; enrichment; C. jejuni;

    Find related papers by JEL classification:

    References

    References listed on IDEAS
    Please report citation or reference errors to , or , if you are the registered author of the cited work, log in to your RePEc Author Service profile, click on "citations" and make appropriate adjustments.:
    as in new window
    1. World Bank & Food and Agriculture Organization & International Fund for Agricultural Development, 2009. "Gender in Agriculture Sourcebook," World Bank Publications, The World Bank, number 6603, January.
    Full references (including those not matched with items on IDEAS)

    Citations

    Lists

    This item is not listed on Wikipedia, on a reading list or among the top items on IDEAS.

    Statistics

    Access and download statistics

    Corrections

    When requesting a correction, please mention this item's handle: RePEc:gam:jagris:v:3:y:2013:i:3:p:579-595:d:28898. See general information about how to correct material in RePEc.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: (XML Conversion Team).

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If references are entirely missing, you can add them using this form.

    If the full references list an item that is present in RePEc, but the system did not link to it, you can help with this form.

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your profile, as there may be some citations waiting for confirmation.

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.